Acknowledgments
We thank Drs. Toshi Nagata and Masato Uchijima of Hamamatsu University School of Medicine for their helpful discussion. M. tuberculosis H37Rv was kindly provided by Dr. Isamu Sugawara of Research Institute of Tuberculosis in Tokyo. This work was supported by Grants-in-Aid for Scientific Research and COE Research from Belinostat Ministry of Education, Culture, Sports, Science and Technology of Japan; by Health and Labour Science Research Grants for Research into Emerging and Reemerging Infectious Diseases from the Ministry of Health, Labour and Welfare of Japan; and by the United States-Japan Cooperative Medical Science Committee.
Appendix A. Supplementary data
Supplemental Fig. 1.
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Supplementary Movie S1.
Live 4D confocal microscopy of Rab7 localization dynamics on an M.tb phagosome. This movie corresponds to Fig. 1E. Raw264.7 cells expressing EGFP-Rab7 were observed from 30 to 155 min after infection of M.tb expressing DsRed. Frames were collected at 5 min intervals.
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