AP-1 was involved in Ang II-induced TGF-β1 expression in dermal fibroblasts. (A) Effect of AP-1 decoy ODNs on Ang II-induced TGF-β1 mRNA. Cells were transfected with AP-1 decoy ODNs (1 μM) or mutated decoy ODNs (1 μM) and then stimulated with Ang II (1 μM) for 24 h. A representative Northern blot analysis of four experiments is shown. (B) Effect of AP-1 decoy on Ang II-induced AP-1 binding. Nuclear proteins were extracted from Ang II (1 μM)-stimulated
AMG 487 transfected with AP-1 decoy or mutated decoy. EMSA was performed to measure AP-1 DNA-binding. The specificity of the binding was determined by preincubation with 100-fold excess of unlabeled probes or with 2 μg of anti-c-Fos Ab. A representative EMSA of four experiments is
Rodinia shown. (C) Effect of AP-1 decoy on exogenous TGF-β1-induced proα1(I) mRNA expression. Cells were transfected with AP-1 decoy ODNs (1 μM) or mutated decoy ODNs (1 μM) and stimulated with TGF-β1 (1 ng/ml) for 24 h. A representative Northern blot of four experiments is shown. (D) Effect of decorin on exogenous TGF-β1-induced proα1(I) mRNA expression. Cells were pretreated with decorin (100 nM) and then stimulated with TGF-β1 (0.5 ng/ml) for 24 h. A representative Northern blot analysis of four experiments is shown.
