Fig. 1.
Anti-inflammatory effects of nifedipine on AGE-exposed mesangial cells. Mesangial PF-562271 were treated with 100 μg/ml AGE-BSA or non-glycated BSA in the presence or absence of 1 μM nifedipine, 1 μM amlodipine and 500 nM GW-9662 for 4 h (A–C) or 24 h (D). Then RAGE gene [removed](A), ROS generation (B), VCAM-1 gene [removed](C) and MCP-1 production (D) were measured. (B) Upper panel shows quantitative data of ROS generation evaluated by fluorescent intensity. Lower panel shows typical microphotographs of the cells. * and **, p cells by suppressing the down-stream signaling induced by AGE–RAGE. For this, we studied the effect of nifedipine on VCAM-1 gene expression and MCP-1 production in AGE-exposed mesangial cells.
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Immunohistochemistry and assessment Antibodies used were as follows IL goat
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