The obvious difference in mitochondrial morphology between normal (IMO) and mdx myoblasts (SC-5) ( Fig. 1) immediately suggest affected mitochondrial functioning in the latter. To verify this, several basic parameters relevant to mitochondria were compared between the two cell lines.
Fig. 1.
Intracellular mitochondrial organization in SC-5 and IMO cells. Confocal images of mitochondria labeled with the MitoTracker CMX Ros.
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Fig. 2.
Oxygen consumption by SC-5 and IMO cells. At the beginning of each assay the respiration rate was measured in SNS-032 suspended in the substrate free solution, then pyruvate, glucose, oligomycin and CCCP were added sequentially (n = 4). * refers to SC-5 vs. IMO; # refers to the effect of glucose. ????p species formation in SC-5 and IMO cells. (A) Mitochondrial membrane potential. Bars reflect the ratio of the orange and green JC-2 fluorescence intensity (n = 4). * refers to SC-5 vs. IMO; # refers to the effect of CCCP. ?p
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