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Protein assay. Protein was measured in Anacetrapib solubilized in 0.5 M NaOH according to Bradford [18] using commercially available Protein Assay Kit (Bio-Rad).
Statistical analysis. Quantitative data are expressed as means ± standard deviations. Statistical significance was calculated using Student’s t-test.
Results and discussion
Mutations in the dystrophin gene result in a broad spectrum of abnormalities, leading to cell malfunctioning and premature death. Among the various cell types, differentiated muscle cells are the most severely affected. The major deficiencies involve calcium homeostasis and energy metabolism [19], [20], [21] and [22]. It is assumed that these defects are caused by a lack of dystrophin proteins. Data shown here clearly indicate that immature dystrophic muscle cells, myoblasts also exhibit severe changes in crucial mitochondrial parameters in comparison to their healthy equivalents. These abnormalities cannot be due to a lack of dystrophin, as it is not present in normal myoblasts either.