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The silence of CMTM5 in cancer cell lines is due to ABT-888 methylation, so we also detected CMTM5 in pancreatic cancer cell lines. MSP showed that all the cell lines were methylated ( Fig. 1B). The detailed methylation status of each individual CpG site was examined by BGS. The results were consistent with those of MSP ( Fig. 1C).
CMTM5 is decreased in pancreatic cancer tissues
Using a pancreatic cancer tissue microarray, we evaluated the expression of CMTM5 protein in samples of normal pancreas (n = 22) and pancreatic adenocarcinoma (n = 57). The level of expression of CMTM5 in individual samples was scored as negative, weakly positive, and positive by a blinded pathologist. The expression of CMTM5 was observed primarily in the cytoplasm ( Fig. 2A). All the normal tissue samples were graded as positive. However, most pancreatic cancer samples were graded as negative (40 of 57 [70%]), whereas 25% were weakly positive ( Fig. 2B).