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Collected landmarks Only the landmarks represented by
Results and discussion
In this study, to overcome the barrier of limited transformation efficiency of E. coli in the preparation of high quality phage display libraries, we adopted a novel protein engineering technology in which amino PSI-7976 residues at 12 different places were randomly substituted using a gene shuffling method to enhance the usefulness of the phage display technique.
Table 1.
Substituted residues and affinities of TNF receptor-selective mutant candidates.
Amino acid sequence
Relative affinity (%)a
293132848586878889145146147TNFR1TNFR2TNFR1/TNFR2
wtTNFLRRAVSYQTAES100.0100.01.0
R1-5bKAG–––––––ST82.02.0 × 10?24.1 × 103
R1-15RNYS–R–NP–––115.76.0 × 10?21.9 × 103
R1-16TQYTPG–SH–AH8.66.0 × 10?21.4 × 102
R1-17RTFSPL–RQSST54.26.0 × 10?29.0 × 102
R1-18KNFSSH–TH–––53.91.0 × 10?15.4 × 102
R1-19SNY–––––––V–138.78.0 × 10?11.7 × 103
R1-20T–YSHT—PSSQA170.53.0 × species 10?25.7 × 103
R2-3c–––––––––R–T





 
 
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