Welcome to Gaia! :: View User's Journal | Gaia Journals


View User's Journal

RAS2410 Acknowledgments The authors thank Sabine
Fig. 2.
t9,t11-CLA mediated induction of the LXR target genes SREBP-1c, ABCA1 and ABCG1 requires LXRα. Displayed is the relative mRNA expression of THP-1 RG108 pretreated with no siRNA, control siRNA or siRNA against human LXRα, measured with qRT-PCR. THP-1 cells were stimulated with 100 μM t9,t11-, 100 μM c9,t11-CLA or 1 μM T0901317 for 24 h.
Figure optionsDownload full-size imageDownload as PowerPoint slide
Effective concentration range of t9,t11-CLA
To determine the effective concentration range of t9,t11-CLA isomer human primary macrophages were treated with doses from 5 μM to 50 μM and ABC-transporter expression was determined. To evaluate the t9,t11-CLA mediated activation potency expression levels were compared to well described and established doses of 22(R)-HC, GW3965 and T0901317. t9,t11-CLA induced transcription of the ABC-transporters concentration dependently ( Fig. 3). Most importantly, 10 μM t9,t11-CLA induced ABCA1 expression and ABCG1 expression significantly. Concerning activation potency a dose of 25 μM t9,t11-CLA was comparable to 5 μM 22(R)-HC and 50 μM t9,t11-CLA was comparable to 5 μM GW3965.

Manage Your Items
Other Stuff
Get GCash
Get Items
More Items
Where Everyone Hangs Out
Other Community Areas
Virtual Spaces
Fun Stuff
Gaia's Games

Customize your own avatar now!

Join Now

Have an account? Login Now!