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Density Monthly captures were performed between September
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Fig. 4.
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Systematic gene regulation involving gene silencing during neuronal differentiation
Acknowledgments
This work was supported in part by research grants from VSV-G tag Ministry of Health, Labour and Welfare of Japan and by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science.
Appendix A. Supplementary data
Supplementary data.
Expression profiles of miRNAs. DNA chip analysis with the Genopal?-MICM DNA chips (Mitsubishi Rayon) was carried out. Hybridization signals of the let-7 members and indicated miRNAs are exhibited in undifferentiated and differentiated P19 cells as in Fig. 1. The signals of miR-124a and -125, which are brain-specific and markedly increased in differentiated cells, miR-302 and -295, which are specifically expressed in embryonal stem cells, and then miR-20a and -106b, which appear to be stably expressed over the course of differentiation, are shown.





 
 
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