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It has been reported that glucose regulates the protein stability of insulin and MafA transcription factor in β Nutlin-3 [8] and [21]. We evaluated whether collectrin protein turnover is influenced by glucose level. MIN6 cells were incubated in the presence of cycloheximide, a translational inhibitor, in the presence of 25 mM glucose. During the incubation, the expression level of collectrin protein decayed rapidly compared with that of GAPDH (Fig. 2B), indicating the relative instability of collectrin protein. MIN6 cells were also cultured with cycloheximide under 5.5 or 25 mM glucose. A similar rapid decrease in collectrin protein was found under both concentrations (Fig. 2C), indicating that augmented collectrin protein expression by high glucose is not due to increased collectrin protein stability. Collectively, these results suggest that glucose stimulation of collectrin protein expression is predominantly mediated at a translational level.