Plasmids. pCMX-Nurr1, -Nor1, -NGFI-B, -Nurr1R334A and -Nurr1dim have been described by [21]. The luciferase reporter MH100-tk-luc contains four copies of the GAL4 binding site upstream of the herpes simplex virus thymidine kinase promoter [12]. pCMX-RXRα, -LacZ, -GAL4-Nurr1 and -GAL4-Nurr1dim are described by [22]. PPREx3-tk-luc has been described by [23], while PSG5-PPARβ/δ was provided by Dr. Desvergne (University of Lausanne, Switzerland). The FABP5 promoter fragment was amplified from genomic DNA extracted from HEK293
LY 317615 using QuickExtract DNA extraction solution (Epicentre Biotechnologies). The following primers were used: 5′-AAAAGCTAGCGTGGTCTGATTTCATAAGGT-3′ and 5′-AAAAAGATCTGCACCCGGCGCCGGCGGCTG-3′. The PCR-amplified promoter fragment was ligated into the pGL2 basic plasmid (Promega). The FABP5 promoter construct with the mutated NBRE site was generated with a Pfu ultra polymerase (Stratagene) reaction with the wild-type promoter construct as a template and the following primers: F: 5′-GCGAGGAGCAGAAGGAAAGGGAGGCACCGTAG-3′ and R: 5′-CTACGGTGCCTCCCTTTCCTTCTGCTCCTCGC-3′. The human FABP5 cDNA was amplified from the IRAVp968H094 cDNA
clone (RZPD, Accession No. BC00200
cool using the following primers: 5′-AAAAAAGCTTATGGCCAGTCTTAAGGATCT-3′ and 5′-AAAAGCTAGCTCATTGCACCTTCTCATAGA-3′. The PCR-amplified fragment was cloned into the pCMX vector.
