Welcome to Gaia! :: View User's Journal | Gaia Journals

Figure optionsDownload full-size imageDownload as PowerPoint slide
Fig. 2.
THP-1 derived PP1 src were treated with TNFα (10 ng/ml) for the indicated times in the absence (A and C) or presence (B and D) of the channel blocker SKF96365 (SKF, 30 μM, 15 min pre-treatment) and processed for immunodetection of phospho-AKT (A and B) or phospho-BAD (C and D) in whole cell lysates. Membranes were reprobed for total AKT (A), total BAD (C) or GAPDH (B and D) to control for protein loading. Blots are representative from at least three independent experiments.
Figure optionsDownload full-size imageDownload as PowerPoint slide
Fig. 3.
THP-1 derived macrophages were grown on Lab-Tek chambers and exposed for 24 h (37 °C in humidified air/5% CO2 atmosphere) to complete growth medium (“CM”) or serum-free medium (“RPMI”), with or without TNFα (10 ng/ml). Alternatively, macrophages were exposed to TNFα (10 ng/ml) in complete growth medium but in the presence of the channel blocker SKF96365 (SKF, 30 μM). In all instances, macrophages were then processed for evaluation of apoptosis by TUNEL assay, as described in Methods. ?P