Spectrophotometer help...

[i HAVE AiDZ]

Why does the amount of absorption change with different wavelengths of light?

[DXnobodyX]

http://en.wikipedia.org/wiki/Resonance

Basically the amount of energy transfered to a molecule is dependent on wavelength and the size of the molecule, plus other things that aren't so important. So changing the wavelength would change how much energy is absorbed by the sample and thus the end result, also changing the sample but using the same wavelength will change the end result.

[i HAVE AiDZ]

DXnobodyX

http://en.wikipedia.org/wiki/Resonance

Basically the amount of energy transfered to a molecule is dependent on wavelength and the size of the molecule, plus other things that arent so important. So changing the wavelength would change how much energy is absorbed by the sample and thus the end result, also changing the sample but using the same wavelength will change the end result.

Thank you!!! smile